Introgression of Swertia mussotii gene into Bupleurum scorzonerifolium via somatic hybridization.

ABSTRACT:

Background

The wild herb Swertia mussotii is a source of the anti-hepatitis compounds swertiamarin, mangiferin and gentiopicroside. Its over-exploitation has raised the priority of producing these compounds heterologously. Somatic hybridization represents a novel approach for introgressing Swertia mussotii genes into a less endangered species.

Results

Protoplasts derived from calli of Bupleurum scorzonerifolium and S. mussotii were fused to produce 194 putative hybrid cell lines, of which three (all derived from fusions where the S. mussotii protoplasts were pre-treated for 30 s with UV light) later differentiated into green plants. The hybridity of the calli was confirmed by a combination of isozyme, RAPD and chromosomal analysis. The hybrid calli genomes were predominantly

SUBMITTER: Wang J 

PROVIDER: S-EPMC3098146 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature