Rapid screening of complex DNA samples by single-molecule amplification and sequencing.

ABSTRACT: Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.

SUBMITTER: Huang J 

PROVIDER: S-EPMC3098247 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature