Differential regulation of PDE5 expression in left and right ventricles of feline hypertrophy models.

ABSTRACT:

Background

Though long known to affect smooth muscle biology, recent studies indicate that phosphodiesterase 5 (PDE5) is also expressed in myocardium. Recognizing that the regulation of PDE5 in hypertrophy is not well understood, we assessed the response of PDE5 expression and the level of cGMP-dependent kinase I (cGKI) in the left and right ventricles of feline hypertrophy models.

Methodology/principal findings

Using a cDNA library of feline aortic smooth muscle cells, we identified and cloned PDE5 cDNA for the first time in this species. The sequence shares 98% identity with its human orthologue at the amino acid level. E. coli expression of the cloned allele allowed selection of antibodies with appropriate specificity, facilitating the analysis of PDE5 expression in felin

SUBMITTER: Shan X 

PROVIDER: S-EPMC3098252 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature