Visual genome-wide RNAi screening to identify human host factors required for Trypanosoma cruzi infection.

ABSTRACT: The protozoan parasite Trypanosoma cruzi is the etiologic agent of Chagas disease, a neglected tropical infection that affects millions of people in the Americas. Current chemotherapy relies on only two drugs that have limited efficacy and considerable side effects. Therefore, the development of new and more effective drugs is of paramount importance. Although some host cellular factors that play a role in T. cruzi infection have been uncovered, the molecular requirements for intracellular parasite growth and persistence are still not well understood. To further study these host-parasite interactions and identify human host factors required for T. cruzi infection, we performed a genome-wide RNAi screen using cellular microarrays of a printed siRNA library that spanned the whole human genom

SUBMITTER: Genovesio A 

PROVIDER: S-EPMC3098829 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature