A rapid, extensive, and transient transcriptional response to estrogen signaling in breast cancer cells.

ABSTRACT: We report the immediate effects of estrogen signaling on the transcriptome of breast cancer cells using global run-on and sequencing (GRO-seq). The data were analyzed using a new bioinformatic approach that allowed us to identify transcripts directly from the GRO-seq data. We found that estrogen signaling directly regulates a strikingly large fraction of the transcriptome in a rapid, robust, and unexpectedly transient manner. In addition to protein-coding genes, estrogen regulates the distribution and activity of all three RNA polymerases and virtually every class of noncoding RNA that has been described to date. We also identified a large number of previously undetected estrogen-regulated intergenic transcripts, many of which are found proximal to estrogen receptor binding sites. Collecti

SUBMITTER: Hah N 

PROVIDER: S-EPMC3099127 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature