Molecular characterization of community structures and sulfur metabolism within microbial streamers in Japanese hot springs.

ABSTRACT: Community structures of submerged microbial slime streamers (SMSS) in sulfide-containing hot springs at 72 to 80 degrees C at Nakabusa and Yumata, Japan, were investigated by molecular analysis based on the 16S rRNA gene. The SMSS were classified into two consortia; consortium I occurred at lower levels of sulfide in the hot springs (less than 0.1 mM), and consortium II dominated when the sulfide levels were higher (more than 0.1 mM). The dominant cell morphotypes in consortium I were filamentous and small rod-shaped cells. The filamentous cells hybridized with fluorescent oligonucleotide probes for the domain Bacteria, the domain Archaea, and the family Aquificaceae: Our analysis of the denaturing gradient gel electrophoresis (DGGE) bands by using reverse transcription (RT)-PCR amplificat

SUBMITTER: Nakagawa T 

PROVIDER: S-EPMC309991 | biostudies-literature | 2003 Dec

REPOSITORIES: biostudies-literature