Proteomic characterization of human plasma high density lipoprotein fractionated by gel filtration chromatography.

ABSTRACT: Plasma levels of high density lipoprotein cholesterol (HDL-C) are inversely proportional to the incidence of cardiovascular disease. Recent applications of modern proteomic technologies have identified upward of 50 distinct proteins associated with HDL particles with many of these newly discovered proteins implicating HDL in nonlipid transport processes including complement activation, acute phase response and innate immunity. However, almost all MS-based proteomic studies on HDL to date have utilized density gradient ultracentrifugation techniques for HDL isolation prior to analysis. These involve high shear forces and salt concentrations that can disrupt HDL protein interactions and alter particle function. Here, we used high-resolution size exclusion chromatography to fractionate normal

SUBMITTER: Gordon SM 

PROVIDER: S-EPMC3100175 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature