Rapid identification of allergenic and pathogenic molds in environmental air by an oligonucleotide array.

ABSTRACT:

Background

Airborne fungi play an important role in causing allergy and infections in susceptible people. Identification of these fungi, based on morphological characteristics, is time-consuming, expertise-demanding, and could be inaccurate.

Methods

We developed an oligonucleotide array that could accurately identify 21 important airborne fungi (13 genera) that may cause adverse health problems. The method consisted of PCR amplification of the internal transcribed spacer (ITS) regions, hybridization of the PCR products to a panel of oligonucleotide probes immobilized on a nylon membrane, and detection of the hybridization signals with alkaline phosphatase-conjugated antibodies.

Results

A collection of 72 target and 66 nontarget reference strains were analyzed by the a

SUBMITTER: Hung WT 

PROVIDER: S-EPMC3100263 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature