Methylation profile of single hepatocytes derived from hepatitis B virus-related hepatocellular carcinoma.

ABSTRACT:

Background

With the development of high-throughput screening, a variety of genetic alterations has been found in hepatocellular carcinoma (HCC). Although previous studies on HCC methylation profiles have focused on liver tissue, studies using isolated hepatocytes are rare. The heterogeneity of liver composition may impact the genuine methylation status of HCC; therefore, it is important to clarify the methylation profile of hepatocytes to aid in understanding the process of tumorigenesis.

Methods and findings

The global methylation profile of single hepatocytes isolated from liver tissue of hepatitis B virus (HBV) related HCC (HBHC) was analyzed using Illumina Infinium Human Methylation27 BeadChips, and combined bisulfite restriction analysis (COBRA) and bisulfite sequencing

SUBMITTER: Tao R 

PROVIDER: S-EPMC3100314 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature