Estrogen-initiated transformation of prostate epithelium derived from normal human prostate stem-progenitor cells.

ABSTRACT: The present study sought to determine whether estrogens with testosterone support are sufficient to transform the normal human prostate epithelium and promote progression to invasive adenocarcinoma using a novel chimeric prostate model. Adult prostate stem/early progenitor cells were isolated from normal human prostates through prostasphere formation in three-dimensional culture. The stem/early progenitor cell status and clonality of prostasphere cells was confirmed by immunocytochemistry and Hoechst staining. Normal prostate progenitor cells were found to express estrogen receptor α, estrogen receptor β, and G protein-coupled receptor 30 mRNA and protein and were responsive to 1 nm estradiol-17β with increased numbers and prostasphere size, implicating them as direct estrogen targets. Rec

SUBMITTER: Hu WY 

PROVIDER: S-EPMC3100619 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature