Topoisomerase IIbeta is required for lamina-specific targeting of retinal ganglion cell axons and dendrites.

ABSTRACT: The specific partnering of synaptically connected neurons is central to nervous system function. Proper wiring requires the interchange of signals between a postmitotic neuron and its environment, a distinct pattern of transcription in the nucleus, and deployment of guidance and adhesion cues to the cell surface. To identify genes involved in neurite targeting by retinal ganglion cells (GCs), their presynaptic partners in the retina, and their postsynaptic targets in the optic tectum, we undertook a forward genetic screen for mutations disrupting visual responses in zebrafish. This rapid primary screen was subsequently refined by immunohistochemical labeling of retinal and tectal neurites to detect patterning errors. From this unbiased screen, the notorious (noto) mutant exhibited the most

SUBMITTER: Nevin LM 

PROVIDER: S-EPMC3100707 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature