Comprehensive DNA methylation analysis of human peripheral blood leukocytes and lymphoblastoid cell lines.

ABSTRACT: DNA methylation is involved in development and in human diseases. Genomic DNA derived from lymphoblastoid cell lines (LCLs) is commonly used to study DNA methylation. There are potential confounding factors regarding the use of LCL-derived DNA, however, such as Epstein-Barr (EB) viral infection and artifacts induced during cell culture. Recently, several groups compared the DNA methylation status of peripheral blood leukocytes (PBLs) and LCLs and concluded that the DNA methylation profiles between them might be consistent. To confirm and extend theses results, we performed a comprehensive DNA methylation analysis using both PBLs and LCLs derived from the same individuals. Using the luminometric methylation assay, we revealed that the global DNA methylation level was different between PBLs

SUBMITTER: Sugawara H 

PROVIDER: S-EPMC3100768 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature