RNA 3' processing functions of Arabidopsis FCA and FPA limit intergenic transcription.

ABSTRACT: The RNA-binding proteins FCA and FPA were identified based on their repression of the flowering time regulator FLC but have since been shown to have widespread roles in the Arabidopsis thaliana genome. Here, we use whole-genome tiling arrays to show that a wide spectrum of genes and transposable elements are misexpressed in the fca-9 fpa-7 (fcafpa) double mutant at two stages of seedling development. There was a significant bias for misregulated genomic segments mapping to the 3' region of genes. In addition, the double mutant misexpressed a large number of previously unannotated genomic segments corresponding to intergenic regions. We characterized a subset of these misexpressed unannotated segments and established that they resulted from extensive transcriptional read-through, use of dow

SUBMITTER: Sonmez C 

PROVIDER: S-EPMC3100917 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature