Hesperedin promotes MyoD-induced myogenic differentiation in vitro and in vivo.

ABSTRACT:

Background and purpose

The bioflavonoid, hesperedin, promotes osteoblast differentiation in human mesenchymal stem cells, indicating an anabolic effect of hesperedin on bone metabolism. Murine bone marrow mesenchymal stem cells undergo myogenic differentiation as well as osteogenic differentiation. We therefore explored whether hesperedin modulates muscle cell differentiation.

Experimental approach

Myoblast C2C12 cells were differentiated into muscle cells in the presence or absence of hesperedin. The effects of hesperedin on myogenic differentiation were determined by analysing specific muscle markers in vitro using reporter gene assays, immunoblotting, RT-PCR and DNA pull-down assays. In vivo, the effects of hesperedin were assessed using the freeze injury-induced muscle r

SUBMITTER: Jeong H 

PROVIDER: S-EPMC3101621 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature