Early gene expression changes in the retinal ganglion cell layer of a rat glaucoma model.

ABSTRACT:

Purpose

To identify patterns of early gene expression changes in the retinal ganglion cell layer (GCL) of a rodent model of chronic glaucoma.

Methods

Prolonged elevation of intraocular pressure (IOP) was produced in rats by episcleral vein injection of hypertonic saline (N = 30). GCLs isolated by laser capture microdissection were grouped by grading of the nerve injury (<25% axon degeneration for early injury; >25% for advanced injury). Gene expression was determined by cDNA microarray of independent GCL RNA samples. Quantitative PCR (qPCR) was used to further examine the expression of selected genes.

Results

By array analysis, 533 GCL genes (225 up, 308 down) were significantly regulated in early injury. Compared to only one major upregulated gene class of metabolism

SUBMITTER: Guo Y 

PROVIDER: S-EPMC3101663 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature