Proteomic analyses of corneal tissue subjected to alkali exposure.

ABSTRACT:

Purpose

To determine whether exposure to alkaline chemicals results in predictable changes in corneal protein profile. To determine whether protein profile changes are indicative of severity and duration of alkali exposure.

Methods

Enucleated bovine and porcine (n = 59 each) eyes were used for exposure to sodium, ammonium, and calcium hydroxide, respectively. Eyes were subjected to fluorescein staining, 5-bromo-2'-deoxy-uridine (BrdU) labeling. Excised cornea was subjected to protein extraction, spectrophotometric determination of protein amount, dynamic light scattering and SDS-PAGE profiling, mass spectrometric protein identification, and iTRAQ-labeled quantification. Select identified proteins were subjected to Western blot and immunohistochemical analyses.

Results

SUBMITTER: Parikh T 

PROVIDER: S-EPMC3101685 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature