Identification of B cell epitopes of alcohol dehydrogenase allergen of Curvularia lunata.

ABSTRACT:

Background/objective

Epitope identification assists in developing molecules for clinical applications and is useful in defining molecular features of allergens for understanding structure/function relationship. The present study was aimed to identify the B cell epitopes of alcohol dehydrogenase (ADH) allergen from Curvularia lunata using in-silico methods and immunoassay.

Method

B cell epitopes of ADH were predicted by sequence and structure based methods and protein-protein interaction tools while T cell epitopes by inhibitory concentration and binding score methods. The epitopes were superimposed on a three dimensional model of ADH generated by homology modeling and analyzed for antigenic characteristics. Peptides corresponding to predicted epitopes were synthesized and im

SUBMITTER: Nair S 

PROVIDER: S-EPMC3102081 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature