An adaptable method using human mixed tissue ratiometric controls for benchmarking performance on gene expression microarrays in clinical laboratories.

ABSTRACT:

Background

Molecular biomarkers that are based on mRNA transcripts are being developed for the diagnosis and treatment of a number of diseases. DNA microarrays are one of the primary technologies being used to develop classifiers from gene expression data for clinically relevant outcomes. Microarray assays are highly multiplexed measures of comparative gene expression but have a limited dynamic range of measurement and show compression in fold change detection. To increase the clinical utility of microarrays, assay controls are needed that benchmark performance using metrics that are relevant to the analysis of genomic data generated with biological samples.

Results

Ratiometric controls were prepared from commercial sources of high quality RNA from human tissues with distinc

SUBMITTER: Pine PS 

PROVIDER: S-EPMC3103427 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature