Overlapping repressor binding sites regulate expression of the Methanococcus maripaludis glnK(1) operon.

ABSTRACT: The euryarchaeal transcriptional repressor NrpR regulates a variety of nitrogen assimilation genes by 2-oxoglutarate-reversible binding to conserved palindromic operators. The number and positioning of these operators varies among promoter regions of regulated genes, suggesting NrpR can bind in different patterns. Particularly intriguing is the contrast between the nif and glnK(1) promoter regions of Methanococcus maripaludis, where two operators are present but with different configurations. Here we study NrpR binding and regulation at the glnK(1) promoter, where the two operator sequences overlap and occur on opposite faces of the double helix. We find that both operators function in binding, with a dimer of NrpR binding simultaneously to each overlapping operator. We show in vivo that t

SUBMITTER: Lie TJ 

PROVIDER: S-EPMC3104677 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature