Detection and analysis of endogenous badnaviruses in the New Zealand flora.

ABSTRACT:

Background and aims

Badnaviruses and their host-integrated DNA occur in tropical crops and a few northern temperate species. Following the discovery of a badnavirus on a subantarctic island with floristic links to New Zealand, we postulated that badnaviruses exist in the New Zealand flora. Badnavirus reverse transcriptase (RT) sequences consist of variable regions flanked by highly conserved regions. This study used RT sequences to detect and characterize badnavirus sequences in the New Zealand flora and to investigate their utility for the study of broader aspects of plant biology.

Methodology

Molecular diversity of RT sequences was analysed using polymerase chain reaction and denaturing gradient gel electrophoresis (DGGE). In a study of the genus Melicytus, internal transc

SUBMITTER: Lyttle DJ 

PROVIDER: S-EPMC3104934 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature