Dual organism transcriptomics of airway epithelial cells interacting with conidia of Aspergillus fumigatus.

ABSTRACT:

Background

Given the complex nature of the responses that can occur in host-pathogen interactions, dual transcriptomics offers a powerful method of elucidating these interactions during infection. The gene expression patterns of Aspergillus fumigatus conidia or host cells have been reported in a number of previous studies, but each focused on only one of the interacting organisms. In the present study, we profiled simultaneously the transcriptional response of both A. fumigatus and human airway epithelial cells (AECs).

Methodology

16HBE14o- transformed bronchial epithelial cells were incubated with A. fumigatus conidia at 37°C for 6 hours, followed by genome-wide transcriptome analysis using human and fungal microarrays. Differentially expressed gene lists were generated fro

SUBMITTER: Oosthuizen JL 

PROVIDER: S-EPMC3105077 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature