4D super-resolution microscopy with conventional fluorophores and single wavelength excitation in optically thick cells and tissues.

ABSTRACT:

Background

Optical super-resolution imaging of fluorescently stained biological samples is rapidly becoming an important tool to investigate protein distribution at the molecular scale. It is therefore important to develop practical super-resolution methods that allow capturing the full three-dimensional nature of biological systems and also can visualize multiple protein species in the same sample.

Methodology/principal findings

We show that the use of a combination of conventional near-infrared dyes, such as Alexa 647, Alexa 680 and Alexa 750, all excited with a 671 nm diode laser, enables 3D multi-colour super-resolution imaging of complex biological samples. Optically thick samples, including human tissue sections, cardiac rat myocytes and densely grown neuronal cultures

SUBMITTER: Baddeley D 

PROVIDER: S-EPMC3105105 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature