Multiplex cDNA quantification method that facilitates the standardization of gene expression data.

ABSTRACT: Microarray-based gene expression measurement is one of the major methods for transcriptome analysis. However, current microarray data are substantially affected by microarray platforms and RNA references because of the microarray method can provide merely the relative amounts of gene expression levels. Therefore, valid comparisons of the microarray data require standardized platforms, internal and/or external controls and complicated normalizations. These requirements impose limitations on the extensive comparison of gene expression data. Here, we report an effective approach to removing the unfavorable limitations by measuring the absolute amounts of gene expression levels on common DNA microarrays. We have developed a multiplex cDNA quantification method called GEP-DEAN (Gene expression

SUBMITTER: Gotoh O 

PROVIDER: S-EPMC3105393 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature