Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics.

ABSTRACT:

Background

High abundance protein depletion is a major challenge in the study of serum/plasma proteomics. Prior to this study, most commercially available kits for depletion of highly abundant proteins had only been tested and evaluated in adult serum/plasma, while the depletion efficiency on umbilical cord serum/plasma had not been clarified. Structural differences between some adult and fetal proteins (such as albumin) make it likely that depletion approaches for adult and umbilical cord serum/plasma will be variable. Therefore, the primary purposes of the present study are to investigate the efficiencies of several commonly-used commercial kits during high abundance protein depletion from umbilical cord serum and to determine which kit yields the most effective and reproducible

SUBMITTER: Liu B 

PROVIDER: S-EPMC3105942 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature