Sequence characteristics of T4-like bacteriophage IME08 benome termini revealed by high throughput sequencing.

ABSTRACT:

Background

T4 phage is a model species that has contributed broadly to our understanding of molecular biology. T4 DNA replication and packaging share various mechanisms with human double-stranded DNA viruses such as herpes virus. The literature indicates that T4-like phage genomes have permuted terminal sequences, and are generated by a DNA terminase in a sequence-independent manner;

Methods

genomic DNA of T4-like bacteriophage IME08 was subjected to high throughput sequencing, and the read sequences with extraordinarily high occurrences were analyzed;

Results

we demonstrate that both the 5' and 3' termini of the IME08 genome starts with base G or A. The presence of a consensus sequence TTGGA|G around the breakpoint of the high frequency read sequences suggests that t

SUBMITTER: Jiang X 

PROVIDER: S-EPMC3105952 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature