Capillary electrophoretic analysis reveals subcellular binding between individual mitochondria and cytoskeleton.

ABSTRACT: Interactions between the cytoskeleton and mitochondria are essential for normal cellular function. An assessment of such interactions is commonly based on bulk analysis of mitochondrial and cytoskeletal markers present in a given sample, which assumes complete binding between these two organelle types. Such measurements are biased because they rarely account for nonbound "free" subcellular species. Here we report on the use of capillary electrophoresis with dual laser induced fluorescence detection (CE-LIF) to identify, classify, count, and quantify properties of individual binding events of the mitochondria and cytoskeleton. Mitochondria were fluorescently labeled with DsRed2 while F-actin, a major cytoskeletal component, was fluorescently labeled with Alexa488-phalloidin. In a typical su

SUBMITTER: Kostal V 

PROVIDER: S-EPMC3106115 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature