FR-HIT, a very fast program to recruit metagenomic reads to homologous reference genomes.

ABSTRACT:

Summary

Fragment recruitment, a process of aligning sequencing reads to reference genomes, is a crucial step in metagenomic data analysis. The available sequence alignment programs are either slow or insufficient for recruiting metagenomic reads. We implemented an efficient algorithm, FR-HIT, for fragment recruitment. We applied FR-HIT and several other tools including BLASTN, MegaBLAST, BLAT, LAST, SSAHA2, SOAP2, BWA and BWA-SW to recruit four metagenomic datasets from different type of sequencers. On average, FR-HIT and BLASTN recruited significantly more reads than other programs, while FR-HIT is about two orders of magnitude faster than BLASTN. FR-HIT is slower than the fastest SOAP2, BWA and BWA-SW, but it recruited 1-5 times more reads.

Availability

http://weizhongli-l

SUBMITTER: Niu B 

PROVIDER: S-EPMC3106194 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature