Epistasis between beneficial mutations and the phenotype-to-fitness Map for a ssDNA virus.

ABSTRACT: Epistatic interactions between genes and individual mutations are major determinants of the evolutionary properties of genetic systems and have therefore been well documented, but few quantitative data exist on epistatic interactions between beneficial mutations, presumably because such mutations are so much rarer than deleterious ones. We explored epistasis for beneficial mutations by constructing genotypes with pairs of mutations that had been previously identified as beneficial to the ssDNA bacteriophage ID11 and by measuring the effects of these mutations alone and in combination. We constructed 18 of the 36 possible double mutants for the nine available beneficial mutations. We found that epistatic interactions between beneficial mutations were all antagonistic-the effects of the doub

SUBMITTER: Rokyta DR 

PROVIDER: S-EPMC3107187 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature