Radiation hybrid mapping of the two highly homologous human-variant pMCHL genes by PCR-SSCP.

ABSTRACT: When gene loci are very similar in sequence, as in gene families or multiple pseudogenes, it is difficult to determine the specific location of the individual genes. We show here that applying PCR-SSCP to a radiation hybrid panel allowed mapping and specific sequencing of two genes with only a few sequence differences. Human-variant forms of the promelanin-concentrating hormone (pMCH) gene are found in two locations in the genome, previously localized by FISH to 5p14 and 5q12-q13. Without prior knowledge of sequence variation between the loci, we observed a difference in migration pattern in PCR-SSCP, indicating the presence of at least one point of sequence divergence. PCR-SSCP of 93 samples from a human-hamster radiation hybrid panel revealed the location of the genes to be between marke

SUBMITTER: Miller CL 

PROVIDER: S-EPMC310753 | biostudies-literature | 1998 Jul

REPOSITORIES: biostudies-literature