Maize meiotic mutants with improper or non-homologous synapsis due to problems in pairing or synaptonemal complex formation.

ABSTRACT: During meiotic prophase homologous chromosomes find each other and pair. Then they synapse, as the linear protein core (axial element or lateral element) of each homologous chromosome is joined together by a transverse central element, forming the tripartite synaptonemal complex (SC). Ten uncloned Zea mays mutants in our collection were surveyed by transmission electron microscopy by making silver-stained spreads of SCs to identify mutants with non-homologous synapsis or improper synapsis. To analyse the mutants further, zyp1, the maize orthologue of the Arabidopsis central element component ZYP1 was cloned and an antibody was made against it. Using antibodies against ZYP1 and the lateral element components AFD1 and ASY1, it was found that most mutants form normal SCs but are defective in

SUBMITTER: Golubovskaya IN 

PROVIDER: S-EPMC3107535 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature