Dynamic coinfection with multiple viral subtypes in acute hepatitis C.

ABSTRACT:

Introduction

Acute hepatitis C virus (HCV) infection is rarely studied, but virus sequence evolution and host-virus dynamics during this early stage may influence the outcome of infection. Hypervariable region 1 (HVR1) is genetically diverse and under selective pressure from the host immune response. We analyzed HVR1 evolution by frequent sampling of an acutely infected HCV cohort.

Methods

Three or more pretreatment samples were obtained from each of 10 acutely infected subjects. Polymerase chain reaction amplification was performed with multiple primer combinations to identify the full range of sequences present. Positive samples were cloned and sequenced. Phylogenetic analyses were used to assess viral diversity.

Results

Eight of the 10 subjects were coinfected with

SUBMITTER: Smith JA 

PROVIDER: S-EPMC3107554 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature