Shortening infusion time for high-dose methotrexate alters antileukemic effects: a randomized prospective clinical trial.

ABSTRACT:

Purpose

To determine whether shortening the infusion duration of high-dose methotrexate (HDMTX; 1 g/m(2)) affects the in vivo accumulation of active methotrexate polyglutamates (MTXPG(1-7)) in leukemia cells and whether this differs among major acute lymphoblastic leukemia (ALL) subtypes.

Methods

From June 2000 through October 2007, 356 children with ALL were randomly assigned to receive initial single-agent treatment with HDMTX (1 g/m(2)) as either a 24-hour infusion or a 4-hour infusion at two pediatric hospitals in the United States. The primary outcome measures were the accumulation of MTXPG(1-7) in leukemia cells and the antileukemic effects (eg, inhibition of de novo purine synthesis in bone marrow ALL cells, and decrease in circulating ALL cells).

Results

The 2

SUBMITTER: Mikkelsen TS 

PROVIDER: S-EPMC3107765 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature