Identification of differentially expressed genes in uveal melanoma using suppressive subtractive hybridization.

ABSTRACT:

Purpose

Uveal melanoma (UM) is the most common primary cancer of the eye, resulting not only in vision loss, but also in metastatic death. This study attempts to identify changes in the patterns of gene expression that lead to malignant transformation and proliferation of normal uveal melanocytes (UVM) using the Suppressive Subtractive Hybridization (SSH) technique.

Methods

The SSH technique was used to isolate genes that are differentially expressed in the TP31 cell line derived from a primary UM compared to UVM. The expression level of selected genes was further validated by microarray, semi-quantitative RT-PCR and western blot analyses.

Results

Analysis of the subtracted libraries revealed that 37 and 36 genes were, respectively, up- and downregulated in TP31 cells

SUBMITTER: Landreville S 

PROVIDER: S-EPMC3107995 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature