Silencing survivin splice variant 2B leads to antitumor activity in taxane--resistant ovarian cancer.

ABSTRACT:

Purpose

To study the role of survivin and its splice variants in taxane-resistant ovarian cancer.

Experimental design

We assessed the mRNA levels of survivin splice variants in ovarian cancer cell lines and ovarian tumor samples. siRNAs targeting survivin were designed to silence all survivin splice variants (T-siRNA) or survivin 2B (2B-siRNA) in vitro and orthotopic murine models of ovarian cancer. The mechanism of cell death was studied in taxane-resistant ovarian cancer cells and in tumor sections obtained from different mouse tumors.

Results

Taxane-resistant ovarian cancer cells express higher survivin mRNA levels than their taxane-sensitive counterparts. Survivin 2B expression was significantly higher in taxane-resistant compared with -sensitive cells. Silencing

SUBMITTER: Vivas-Mejia PE 

PROVIDER: S-EPMC3108184 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature