Gene expression pattern in swine neutrophils after lipopolysaccharide exposure: a time course comparison.

ABSTRACT:

Background

Experimental exposure of swine neutrophils to bacterial lipopolysaccharide (LPS) represents a model to study the innate immune response during bacterial infection. Neutrophils can effectively limit the infection by secreting lipid mediators, antimicrobial molecules and a combination of reactive oxygen species (ROS) without new synthesis of proteins. However, it is known that neutrophils can modify the gene expression after LPS exposure. We performed microarray gene expression analysis in order to elucidate the less known transcriptional response of neutrophils during infection.

Methods

Blood samples were collected from four healthy Iberian pigs and neutrophils were isolated and incubated during 6, 9 and 18 hrs in presence or absence of lipopolysaccharide (LPS) fro

SUBMITTER: Sanz-Santos G 

PROVIDER: S-EPMC3108205 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature