A 7.5 Mb sequence-ready PAC contig and gene expression map of human chromosome 11p13-p14.1.

ABSTRACT: The region p13 of the short arm of human chromosome 11 has been studied intensely during the search for genes involved in the etiology of the Wilms' tumor, aniridia, genitourinary abnormalities, mental retardation (WAGR) syndrome, and related conditions. The gene map for this region is far from being complete, however, strengthening the need for additional gene identification efforts. We describe the extension of an existing contig map with P1-derived artificial chromosomes (PACs) to cover 7.5 Mb of 11p13-14.1. The extended sequence-ready contig was established by end probe walking and fingerprinting and consists of 201 PAC clones. Utilizing bins defined by overlapping PACs, we generated a detailed gene map containing 20 genes as well as 22 anonymous ESTs which have been identified by sear

SUBMITTER: Gawin B 

PROVIDER: S-EPMC310838 | biostudies-literature | 1999 Nov

REPOSITORIES: biostudies-literature