Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics.

ABSTRACT: Modern biomedical research greatly benefits from large-scale genome-sequencing projects ranging from studies of viruses, bacteria, and yeast to multicellular organisms, like Caenorhabditis elegans. Comparative genomic studies offer a vast array of prospects for identification and functional annotation of human ortholog genes. We presented a novel comparative proteomic approach for assembling human gene contigs and assisting gene discovery. The C. elegans proteome was used as an alignment template to assist in novel human gene identification from human EST nucleotide databases. Among the available 18,452 C. elegans protein sequences, our results indicate that at least 83% (15,344 sequences) of C. elegans proteome has human homologous genes, with 7,954 records of C. elegans proteins matching

SUBMITTER: Lai CH 

PROVIDER: S-EPMC310876 | biostudies-literature | 2000 May

REPOSITORIES: biostudies-literature