Insights from ANA-grade angiosperms into the early evolution of CUP-SHAPED COTYLEDON genes.

ABSTRACT:

Background and aims

The closely related NAC family genes NO APICAL MERISTEM (NAM) and CUP-SHAPED COTYLEDON3 (CUC3) regulate the formation of boundaries within and between plant organs. NAM is post-transcriptionally regulated by miR164, whereas CUC3 is not. To gain insight into the evolution of NAM and CUC3 in the angiosperms, we analysed orthologous genes in early-diverging ANA-grade angiosperms and gymnosperms.

Methods

We obtained NAM- and CUC3-like sequences from diverse angiosperms and gymnosperms by a combination of reverse transcriptase PCR, cDNA library screening and database searching, and then investigated their phylogenetic relationships by performing maximum-likelihood reconstructions. We also studied the spatial expression patterns of NAM, CUC3 and MIR164 ortholog

SUBMITTER: Vialette-Guiraud AC 

PROVIDER: S-EPMC3108802 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature