Structural organization and expression pattern of the canine RPGRIP1 isoforms in retinal tissue.

ABSTRACT:

Purpose

To examine the structure and expression of RPGRIP1 in dog retina.

Methods

Determination of the structural analysis and expression pattern of canine RPGRIP1 (cRPGRIP1) was based on cDNA amplification. Absolute quantification of the expression level of cRPGRIP1 splice variants was determined by qRT-PCR. Regulatory structures were examined by computational analysis of comparative genomics.

Results

cRPGRIP1 encompasses 25 exons that harbor a 3627-bp open reading frame (ORF) encoding a 1209-amino-acid (aa)-predicted protein. In addition to the main transcript, five full-length and several partial cRPGRIP1 isoforms were identified revealing four alternative 3'-terminal exons--24, 19a, 19c, and 19d--three of which could potentially produce C-terminally truncated prot

SUBMITTER: Kuznetsova T 

PROVIDER: S-EPMC3109012 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature