Expression profiling by iAFLP: A PCR-based method for genome-wide gene expression profiling.

ABSTRACT: The availability of comprehensive sets of genes has prompted the researchers to carry out systematic collection of gene expression data. RT-PCR has the highest specificity and sensitivity for transcript detection among all available methods. Low throughput, especially when quantitative data are desired, has precluded RT-PCR from genome-wide application. Here we report a PCR-based expression profiling method, introduced amplified fragment length polymorphism (iAFLP), that has the same specificity and sensitivity as RT-PCR and a throughput level comparable to that of DNA-microarray hybridization. In this method, restricted ends of total cDNAs from six sources were ligated with adaptors having various length of short insertions to a common sequence (polymorphic adaptors). Amplification of a p

SUBMITTER: Kawamoto S 

PROVIDER: S-EPMC311005 | biostudies-literature | 1999 Dec

REPOSITORIES: biostudies-literature