Patterns and mechanisms of ancestral histone protein inheritance in budding yeast.

ABSTRACT: Replicating chromatin involves disruption of histone-DNA contacts and subsequent reassembly of maternal histones on the new daughter genomes. In bulk, maternal histones are randomly segregated to the two daughters, but little is known about the fine details of this process: do maternal histones re-assemble at preferred locations or close to their original loci? Here, we use a recently developed method for swapping epitope tags to measure the disposition of ancestral histone H3 across the yeast genome over six generations. We find that ancestral H3 is preferentially retained at the 5' ends of most genes, with strongest retention at long, poorly transcribed genes. We recapitulate these observations with a quantitative model in which the majority of maternal histones are reincorporated within

SUBMITTER: Radman-Livaja M 

PROVIDER: S-EPMC3110181 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature