Cloning and characterization of a putative TAC1 ortholog associated with leaf angle in maize (Zea mays L.).

ABSTRACT:

Background

Modifying plant architecture to increase photosynthesis efficiency and reduce shade avoidance response is very important for further yield improvement when crops are grown in high density. Identification of alleles controlling leaf angle in maize is needed to provide insight into molecular mechanism of leaf development and achieving ideal plant architecture to improve grain yield.

Methodology/principal findings

The gene cloning was done by using comparative genomics, and then performing real-time polymerase chain reaction (RT-PCR) analysis to assay gene expression. The gene function was validated by sequence dissimilarity analysis and QTL mapping using a functional cleaved amplified polymorphism (CAP).

Conclusions

The leaf angle is controlled by a major qua

SUBMITTER: Ku L 

PROVIDER: S-EPMC3110200 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature