Assembly, annotation, and integration of UNIGENE clusters into the human genome draft.

ABSTRACT: The recent release of the first draft of the human genome provides an unprecedented opportunity to integrate human genes and their functions in a complete positional context. However, at least three significant technical hurdles remain: first, to assemble a complete and nonredundant human transcript index; second, to accurately place the individual transcript indices on the human genome; and third, to functionally annotate all human genes. Here, we report the extension of the UNIGENE database through the assembly of its sequence clusters into nonredundant sequence contigs. Each resulting consensus was aligned to the human genome draft. A unique location for each transcript within the human genome was determined by the integration of the restriction fingerprint, assembled genomic contig, an

SUBMITTER: Zhuo D 

PROVIDER: S-EPMC311045 | biostudies-literature | 2001 May

REPOSITORIES: biostudies-literature