Molecular characterization of two natural hotspots in the Drosophila buzzatii genome induced by transposon insertions.

ABSTRACT: Transposable elements (TEs) have been implicated in the generation of genetic rearrangements, but their potential to mediate changes in the organization and architecture of host genomes could be even greater than previously thought. Here, we describe the naturally occurring structural and nucleotide variation around two TE insertions in the genome of Drosophila buzzatii. The studied regions correspond to the breakpoints of a widespread chromosomal inversion generated by ectopic recombination between oppositely oriented copies of a TE named Galileo. A detailed molecular analysis by Southern hybridization, PCR amplification, and DNA sequencing of 7.1 kb surrounding the inversion breakpoints in 39 D. buzzatii lines revealed an unprecedented degree of restructuring, consisting of 22 insertions

SUBMITTER: Caceres M 

PROVIDER: S-EPMC311088 | biostudies-literature | 2001 Aug

REPOSITORIES: biostudies-literature