Genetic analysis of H1N1 influenza virus from throat swab samples in a microfluidic system for point-of-care diagnostics.

ABSTRACT: The ability to obtain sequence-specific genetic information about rare target organisms directly from complex biological samples at the point-of-care would transform many areas of biotechnology. Microfluidics technology offers compelling tools for integrating multiple biochemical processes in a single device, but despite significant progress, only limited examples have shown specific, genetic analysis of clinical samples within the context of a fully integrated, portable platform. Herein we present the Magnetic Integrated Microfluidic Electrochemical Detector (MIMED) that integrates sample preparation and electrochemical sensors in a monolithic disposable device to detect RNA-based virus directly from throat swab samples. By combining immunomagnetic target capture, concentration, and purif

SUBMITTER: Ferguson BS 

PROVIDER: S-EPMC3110979 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature