Genome-wide profiling of G protein-coupled receptors in cerebellar granule neurons using high-throughput, real-time PCR.

ABSTRACT:

Background

G protein-coupled receptors (GPCRs) are major players in cell communication, regulate a whole range of physiological functions during development and throughout adult life, are affected in numerous pathological situations, and constitute so far the largest class of drugable targets for human diseases. The corresponding genes are usually expressed at low levels, making accurate, genome-wide quantification of their expression levels a challenging task using microarrays.

Results

We first draw an inventory of all endo-GPCRs encoded in the murine genome. To profile GPCRs genome-wide accurately, sensitively, comprehensively, and cost-effectively, we designed and validated a collection of primers that we used in quantitative RT-PCR experiments. We experimentally validate

SUBMITTER: Maurel B 

PROVIDER: S-EPMC3111393 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature