The transcriptional coregulator RIP140 represses E2F1 activity and discriminates breast cancer subtypes.

ABSTRACT:

Purpose

Receptor-interacting protein of 140 kDa (RIP140) is a transcriptional cofactor for nuclear receptors involved in reproduction and energy homeostasis. Our aim was to investigate its role in the regulation of E2F1 activity and target genes both in breast cancer cell lines and in tumor biopsies.

Experimental design

Glutathione S-transferase pull-down assays, coimmunoprecipitation experiments, and chromatin immunoprecipitation analysis were used to evidence interaction between RIP140 and E2F1. The effects of RIP140 expression on E2F1 activity were determined using transient transfection and quantification of E2F target mRNAs by quantitative real-time PCR. The effect on cell cycle was assessed by fluorescence-activated cell sorting analysis on cells overexpressing green f

SUBMITTER: Docquier A 

PROVIDER: S-EPMC3112174 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature