TILLING for allergen reduction and improvement of quality traits in peanut (Arachis hypogaea L.).

ABSTRACT:

Background

Allergic reactions to peanuts (Arachis hypogaea L.) can cause severe symptoms and in some cases can be fatal, but avoidance is difficult due to the prevalence of peanut-derived products in processed foods. One strategy of reducing the allergenicity of peanuts is to alter or eliminate the allergenic proteins through mutagenesis. Other seed quality traits could be improved by altering biosynthetic enzyme activities. Targeting Induced Local Lesions in Genomes (TILLING), a reverse-genetics approach, was used to identify mutations affecting seed traits in peanut.

Results

Two similar copies of a major allergen gene, Ara h 1, have been identified in tetraploid peanut, one in each subgenome. The same situation has been shown for major allergen Ara h 2. Due to the challeng

SUBMITTER: Knoll JE 

PROVIDER: S-EPMC3113929 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature