ZFN-site searches genomes for zinc finger nuclease target sites and off-target sites.

ABSTRACT:

Background

Zinc Finger Nucleases (ZFNs) are man-made restriction enzymes useful for manipulating genomes by cleaving target DNA sequences. ZFNs allow therapeutic gene correction or creation of genetically modified model organisms. ZFN specificity is not absolute; therefore, it is essential to select ZFN target sites without similar genomic off-target sites. It is important to assay for off-target cleavage events at sites similar to the target sequence.

Results

ZFN-Site is a web interface that searches multiple genomes for ZFN off-target sites. Queries can be based on the target sequence or can be expanded using degenerate specificity to account for known ZFN binding preferences. ZFN off-target sites are outputted with links to genome browsers, facilitating off-target cleavag

SUBMITTER: Cradick TJ 

PROVIDER: S-EPMC3113941 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature