Establishment of a murine epidermal cell line suitable for in vitro and in vivo skin modelling.

ABSTRACT:

Background

Skin diseases are a major health problem. Some of the most severe conditions involve genetic disorders, including cancer. Several of these human diseases have been modelled in genetically modified mice, thus becoming a highly valuable preclinical tool for the treatment of these pathologies. However, development of three-dimensional models of skin using keratinocytes from normal and/or genetically modified mice has been hindered by the difficulty to subculture murine epidermal keratinocytes.

Methods

We have generated a murine epidermal cell line by serially passaging keratinocytes isolated from the back skin of adult mice. We have termed this cell line COCA. Cell culture is done in fully defined media and does not require feeder cells or any other coating methods.<

SUBMITTER: Segrelles C 

PROVIDER: S-EPMC3113952 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature